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    Cell Signaling Technology Inc separate window abca1
    Primers used for real-time qPCR and antibodies used for Western blot analysis
    Separate Window Abca1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 16610 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/separate+window+abca1/pmc07311702-310-92-78?v=Cell+Signaling+Technology+Inc
    Average 99 stars, based on 16610 article reviews
    separate window abca1 - by Bioz Stars, 2026-07
    99/100 stars

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    1) Product Images from "Small molecule inhibition of gut microbial choline trimethylamine lyase activity alters host cholesterol and bile acid metabolism"

    Article Title: Small molecule inhibition of gut microbial choline trimethylamine lyase activity alters host cholesterol and bile acid metabolism

    Journal: American Journal of Physiology - Heart and Circulatory Physiology

    doi: 10.1152/ajpheart.00584.2019

    Primers used for real-time qPCR and antibodies used for Western blot analysis
    Figure Legend Snippet: Primers used for real-time qPCR and antibodies used for Western blot analysis

    Techniques Used: Western Blot

    Primary and secondary antibodies
    Figure Legend Snippet: Primary and secondary antibodies

    Techniques Used:

    Trimethylamine (TMA) lyase inhibition alters the hepatic expression of key genes involved in sterol and bile acid metabolism. At 8 wk of age, wild-type C57BL/6J male mice were switched from standard rodent chow to 1 of 4 experimental synthetic diets containing low (0.02%, wt/wt) or high (0.2%, wt/wt) levels of dietary cholesterol with or without the microbe-targeted TMA lyase inhibitor iodomethylcholine (IMC; 0.06%, wt/wt). After 4 wk on these diets, liver RNA was extracted and gene expression was measured using quantitative real-time PCR. A: relative mRNA expression of sterol regulatory-binding protein 2 (Srebp2), HMG-CoA reductase (HMG CoA-red), HMG-CoA synthase (HMG CoA-syn), squalene synthase (Squalene-syn), ATP-binding cassette transporters (Abca1, Abcg5, and Abcg8), bile acid modifying cytochrome-P450 family members (Cyp7a1, Cyp8b1, and Cyp27a1), bile salt export pump (Bsep), and small heterodimeric partner (Shp) were quantified using the ΔΔCT method. B and C: Western blot analysis HMG-CoA reductase and Cyp7a1 with densitometric quantification. Data shown in A represent the means ± SE for n = 5 mice per group, whereas data in B and C represent the means ± SE for n = 3 mice per group; *significantly different than the nondrug-treated mice within each diet group. AU, arbitrary units.
    Figure Legend Snippet: Trimethylamine (TMA) lyase inhibition alters the hepatic expression of key genes involved in sterol and bile acid metabolism. At 8 wk of age, wild-type C57BL/6J male mice were switched from standard rodent chow to 1 of 4 experimental synthetic diets containing low (0.02%, wt/wt) or high (0.2%, wt/wt) levels of dietary cholesterol with or without the microbe-targeted TMA lyase inhibitor iodomethylcholine (IMC; 0.06%, wt/wt). After 4 wk on these diets, liver RNA was extracted and gene expression was measured using quantitative real-time PCR. A: relative mRNA expression of sterol regulatory-binding protein 2 (Srebp2), HMG-CoA reductase (HMG CoA-red), HMG-CoA synthase (HMG CoA-syn), squalene synthase (Squalene-syn), ATP-binding cassette transporters (Abca1, Abcg5, and Abcg8), bile acid modifying cytochrome-P450 family members (Cyp7a1, Cyp8b1, and Cyp27a1), bile salt export pump (Bsep), and small heterodimeric partner (Shp) were quantified using the ΔΔCT method. B and C: Western blot analysis HMG-CoA reductase and Cyp7a1 with densitometric quantification. Data shown in A represent the means ± SE for n = 5 mice per group, whereas data in B and C represent the means ± SE for n = 3 mice per group; *significantly different than the nondrug-treated mice within each diet group. AU, arbitrary units.

    Techniques Used: Inhibition, Expressing, Real-time Polymerase Chain Reaction, Binding Assay, Western Blot

    Trimethylamine (TMA) lyase inhibition alters the host intestinal expression of key genes involved in sterol and bile acid transport and metabolism. At 8 wk of age, wild-type C57BL/6J male mice were switched from standard rodent chow to 1 of 4 experimental synthetic diets containing low (0.02%, wt/wt) or high (0.2%, wt/wt) levels of dietary cholesterol with or without the microbe-targeted TMA lyase inhibitor iodomethylcholine (IMC; 0.06%, wt/wt). After 4 wk on these diets, RNA was extracted from either the jejunum (A) or ileum (B), and gene expression was measured using quantitative real-time PCR. The mRNA abundance of HMG-CoA reductase (HMG Reduc.), HMG-CoA synthase (HMG Synth.), ATP-binding cassette transporters (Abca1, Abcg5, and Abcg8), Niemann-pick C1-like 1 (Npc1l1), farnesoid X receptor (Fxr), apical sodium bile acid transporter (Asbt), organic solute transporter α and β (Ostα and Ostβ), fibroblast growth factor 15 (Fgf15), and ileal bile acid-binding protein (I-babp) were quantified using the ΔΔCT method. C: Western blot analysis of Abca1, Npc1l1, and Gapdh with densitometric quantification. Data shown in A represent the means ± SE for n = 5 mice per group, whereas data in B and C represent the means ± SE for n = 3 mice per group; *significantly different than the nondrug-treated mice within each diet group.
    Figure Legend Snippet: Trimethylamine (TMA) lyase inhibition alters the host intestinal expression of key genes involved in sterol and bile acid transport and metabolism. At 8 wk of age, wild-type C57BL/6J male mice were switched from standard rodent chow to 1 of 4 experimental synthetic diets containing low (0.02%, wt/wt) or high (0.2%, wt/wt) levels of dietary cholesterol with or without the microbe-targeted TMA lyase inhibitor iodomethylcholine (IMC; 0.06%, wt/wt). After 4 wk on these diets, RNA was extracted from either the jejunum (A) or ileum (B), and gene expression was measured using quantitative real-time PCR. The mRNA abundance of HMG-CoA reductase (HMG Reduc.), HMG-CoA synthase (HMG Synth.), ATP-binding cassette transporters (Abca1, Abcg5, and Abcg8), Niemann-pick C1-like 1 (Npc1l1), farnesoid X receptor (Fxr), apical sodium bile acid transporter (Asbt), organic solute transporter α and β (Ostα and Ostβ), fibroblast growth factor 15 (Fgf15), and ileal bile acid-binding protein (I-babp) were quantified using the ΔΔCT method. C: Western blot analysis of Abca1, Npc1l1, and Gapdh with densitometric quantification. Data shown in A represent the means ± SE for n = 5 mice per group, whereas data in B and C represent the means ± SE for n = 3 mice per group; *significantly different than the nondrug-treated mice within each diet group.

    Techniques Used: Inhibition, Expressing, Real-time Polymerase Chain Reaction, Binding Assay, Western Blot



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    separate window abca1  (Cell Signaling Technology Inc)
    99
    Cell Signaling Technology Inc separate window abca1
    Primers used for real-time qPCR and antibodies used for Western blot analysis
    Separate Window Abca1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/separate+window+abca1/pmc07311702-310-92-78?v=Cell+Signaling+Technology+Inc
    Average 99 stars, based on 1 article reviews
    separate window abca1 - by Bioz Stars, 2026-07
    99/100 stars
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    Primers used for real-time qPCR and antibodies used for Western blot analysis

    Journal: American Journal of Physiology - Heart and Circulatory Physiology

    Article Title: Small molecule inhibition of gut microbial choline trimethylamine lyase activity alters host cholesterol and bile acid metabolism

    doi: 10.1152/ajpheart.00584.2019

    Figure Lengend Snippet: Primers used for real-time qPCR and antibodies used for Western blot analysis

    Article Snippet: Information of antibodies is available in . table ft1 table-wrap mode="anchored" t5 Table 2. caption a7 No. Antibody Vendor Catalog No. Primary antibody information 1 ABCA1 Novus-Biological NB400-105 2 NPC1L1 Gift from Dr. Joyce Repa (Univ. of Texas–Southwestern Medical Center Not applicable 3 HMGCOA-reductase Gift from Dr. Russell DeBose-Boyd (Univ. of Texas–Southwestern Medical Center) Not applicable 4 CYP7A1 Abcam Ab65596 5 Calnexin Cell Signaling Technologies (C5C9)#2679 6 GAPDH Cell Signaling Technologies (D16H11)#8884 Secondary antibody information 1 Anti-rabbit HRP-conjugated Cell Signaling Technologies 7074 2 Anti-mouse HRP-conjugated Cell Signaling Technologies 7076 Open in a separate window ABCA1, ATP-binding cassette transporter; CYP7A1, bile acid modifying cytochrome-P450 family member; NPC1L1, Niemann-pick C1-like 1.

    Techniques: Western Blot

    Primary and secondary antibodies

    Journal: American Journal of Physiology - Heart and Circulatory Physiology

    Article Title: Small molecule inhibition of gut microbial choline trimethylamine lyase activity alters host cholesterol and bile acid metabolism

    doi: 10.1152/ajpheart.00584.2019

    Figure Lengend Snippet: Primary and secondary antibodies

    Article Snippet: Information of antibodies is available in . table ft1 table-wrap mode="anchored" t5 Table 2. caption a7 No. Antibody Vendor Catalog No. Primary antibody information 1 ABCA1 Novus-Biological NB400-105 2 NPC1L1 Gift from Dr. Joyce Repa (Univ. of Texas–Southwestern Medical Center Not applicable 3 HMGCOA-reductase Gift from Dr. Russell DeBose-Boyd (Univ. of Texas–Southwestern Medical Center) Not applicable 4 CYP7A1 Abcam Ab65596 5 Calnexin Cell Signaling Technologies (C5C9)#2679 6 GAPDH Cell Signaling Technologies (D16H11)#8884 Secondary antibody information 1 Anti-rabbit HRP-conjugated Cell Signaling Technologies 7074 2 Anti-mouse HRP-conjugated Cell Signaling Technologies 7076 Open in a separate window ABCA1, ATP-binding cassette transporter; CYP7A1, bile acid modifying cytochrome-P450 family member; NPC1L1, Niemann-pick C1-like 1.

    Techniques:

    Trimethylamine (TMA) lyase inhibition alters the hepatic expression of key genes involved in sterol and bile acid metabolism. At 8 wk of age, wild-type C57BL/6J male mice were switched from standard rodent chow to 1 of 4 experimental synthetic diets containing low (0.02%, wt/wt) or high (0.2%, wt/wt) levels of dietary cholesterol with or without the microbe-targeted TMA lyase inhibitor iodomethylcholine (IMC; 0.06%, wt/wt). After 4 wk on these diets, liver RNA was extracted and gene expression was measured using quantitative real-time PCR. A: relative mRNA expression of sterol regulatory-binding protein 2 (Srebp2), HMG-CoA reductase (HMG CoA-red), HMG-CoA synthase (HMG CoA-syn), squalene synthase (Squalene-syn), ATP-binding cassette transporters (Abca1, Abcg5, and Abcg8), bile acid modifying cytochrome-P450 family members (Cyp7a1, Cyp8b1, and Cyp27a1), bile salt export pump (Bsep), and small heterodimeric partner (Shp) were quantified using the ΔΔCT method. B and C: Western blot analysis HMG-CoA reductase and Cyp7a1 with densitometric quantification. Data shown in A represent the means ± SE for n = 5 mice per group, whereas data in B and C represent the means ± SE for n = 3 mice per group; *significantly different than the nondrug-treated mice within each diet group. AU, arbitrary units.

    Journal: American Journal of Physiology - Heart and Circulatory Physiology

    Article Title: Small molecule inhibition of gut microbial choline trimethylamine lyase activity alters host cholesterol and bile acid metabolism

    doi: 10.1152/ajpheart.00584.2019

    Figure Lengend Snippet: Trimethylamine (TMA) lyase inhibition alters the hepatic expression of key genes involved in sterol and bile acid metabolism. At 8 wk of age, wild-type C57BL/6J male mice were switched from standard rodent chow to 1 of 4 experimental synthetic diets containing low (0.02%, wt/wt) or high (0.2%, wt/wt) levels of dietary cholesterol with or without the microbe-targeted TMA lyase inhibitor iodomethylcholine (IMC; 0.06%, wt/wt). After 4 wk on these diets, liver RNA was extracted and gene expression was measured using quantitative real-time PCR. A: relative mRNA expression of sterol regulatory-binding protein 2 (Srebp2), HMG-CoA reductase (HMG CoA-red), HMG-CoA synthase (HMG CoA-syn), squalene synthase (Squalene-syn), ATP-binding cassette transporters (Abca1, Abcg5, and Abcg8), bile acid modifying cytochrome-P450 family members (Cyp7a1, Cyp8b1, and Cyp27a1), bile salt export pump (Bsep), and small heterodimeric partner (Shp) were quantified using the ΔΔCT method. B and C: Western blot analysis HMG-CoA reductase and Cyp7a1 with densitometric quantification. Data shown in A represent the means ± SE for n = 5 mice per group, whereas data in B and C represent the means ± SE for n = 3 mice per group; *significantly different than the nondrug-treated mice within each diet group. AU, arbitrary units.

    Article Snippet: Information of antibodies is available in . table ft1 table-wrap mode="anchored" t5 Table 2. caption a7 No. Antibody Vendor Catalog No. Primary antibody information 1 ABCA1 Novus-Biological NB400-105 2 NPC1L1 Gift from Dr. Joyce Repa (Univ. of Texas–Southwestern Medical Center Not applicable 3 HMGCOA-reductase Gift from Dr. Russell DeBose-Boyd (Univ. of Texas–Southwestern Medical Center) Not applicable 4 CYP7A1 Abcam Ab65596 5 Calnexin Cell Signaling Technologies (C5C9)#2679 6 GAPDH Cell Signaling Technologies (D16H11)#8884 Secondary antibody information 1 Anti-rabbit HRP-conjugated Cell Signaling Technologies 7074 2 Anti-mouse HRP-conjugated Cell Signaling Technologies 7076 Open in a separate window ABCA1, ATP-binding cassette transporter; CYP7A1, bile acid modifying cytochrome-P450 family member; NPC1L1, Niemann-pick C1-like 1.

    Techniques: Inhibition, Expressing, Real-time Polymerase Chain Reaction, Binding Assay, Western Blot

    Trimethylamine (TMA) lyase inhibition alters the host intestinal expression of key genes involved in sterol and bile acid transport and metabolism. At 8 wk of age, wild-type C57BL/6J male mice were switched from standard rodent chow to 1 of 4 experimental synthetic diets containing low (0.02%, wt/wt) or high (0.2%, wt/wt) levels of dietary cholesterol with or without the microbe-targeted TMA lyase inhibitor iodomethylcholine (IMC; 0.06%, wt/wt). After 4 wk on these diets, RNA was extracted from either the jejunum (A) or ileum (B), and gene expression was measured using quantitative real-time PCR. The mRNA abundance of HMG-CoA reductase (HMG Reduc.), HMG-CoA synthase (HMG Synth.), ATP-binding cassette transporters (Abca1, Abcg5, and Abcg8), Niemann-pick C1-like 1 (Npc1l1), farnesoid X receptor (Fxr), apical sodium bile acid transporter (Asbt), organic solute transporter α and β (Ostα and Ostβ), fibroblast growth factor 15 (Fgf15), and ileal bile acid-binding protein (I-babp) were quantified using the ΔΔCT method. C: Western blot analysis of Abca1, Npc1l1, and Gapdh with densitometric quantification. Data shown in A represent the means ± SE for n = 5 mice per group, whereas data in B and C represent the means ± SE for n = 3 mice per group; *significantly different than the nondrug-treated mice within each diet group.

    Journal: American Journal of Physiology - Heart and Circulatory Physiology

    Article Title: Small molecule inhibition of gut microbial choline trimethylamine lyase activity alters host cholesterol and bile acid metabolism

    doi: 10.1152/ajpheart.00584.2019

    Figure Lengend Snippet: Trimethylamine (TMA) lyase inhibition alters the host intestinal expression of key genes involved in sterol and bile acid transport and metabolism. At 8 wk of age, wild-type C57BL/6J male mice were switched from standard rodent chow to 1 of 4 experimental synthetic diets containing low (0.02%, wt/wt) or high (0.2%, wt/wt) levels of dietary cholesterol with or without the microbe-targeted TMA lyase inhibitor iodomethylcholine (IMC; 0.06%, wt/wt). After 4 wk on these diets, RNA was extracted from either the jejunum (A) or ileum (B), and gene expression was measured using quantitative real-time PCR. The mRNA abundance of HMG-CoA reductase (HMG Reduc.), HMG-CoA synthase (HMG Synth.), ATP-binding cassette transporters (Abca1, Abcg5, and Abcg8), Niemann-pick C1-like 1 (Npc1l1), farnesoid X receptor (Fxr), apical sodium bile acid transporter (Asbt), organic solute transporter α and β (Ostα and Ostβ), fibroblast growth factor 15 (Fgf15), and ileal bile acid-binding protein (I-babp) were quantified using the ΔΔCT method. C: Western blot analysis of Abca1, Npc1l1, and Gapdh with densitometric quantification. Data shown in A represent the means ± SE for n = 5 mice per group, whereas data in B and C represent the means ± SE for n = 3 mice per group; *significantly different than the nondrug-treated mice within each diet group.

    Article Snippet: Information of antibodies is available in . table ft1 table-wrap mode="anchored" t5 Table 2. caption a7 No. Antibody Vendor Catalog No. Primary antibody information 1 ABCA1 Novus-Biological NB400-105 2 NPC1L1 Gift from Dr. Joyce Repa (Univ. of Texas–Southwestern Medical Center Not applicable 3 HMGCOA-reductase Gift from Dr. Russell DeBose-Boyd (Univ. of Texas–Southwestern Medical Center) Not applicable 4 CYP7A1 Abcam Ab65596 5 Calnexin Cell Signaling Technologies (C5C9)#2679 6 GAPDH Cell Signaling Technologies (D16H11)#8884 Secondary antibody information 1 Anti-rabbit HRP-conjugated Cell Signaling Technologies 7074 2 Anti-mouse HRP-conjugated Cell Signaling Technologies 7076 Open in a separate window ABCA1, ATP-binding cassette transporter; CYP7A1, bile acid modifying cytochrome-P450 family member; NPC1L1, Niemann-pick C1-like 1.

    Techniques: Inhibition, Expressing, Real-time Polymerase Chain Reaction, Binding Assay, Western Blot